Variability among Rhizobium Strains Originating from Nodules of Vicia faba

Author:

van Berkum P,Beyene D,Vera F T,Keyser H H

Abstract

Rhizobium strains from nodules of Vicia faba were diverse in plasmid content and serology. Results of multilocus gel electrophoresis and restriction fragment length polymorphism indicated several deep chromosomal lineages among the strains. Linkage disequilibrium among the chromosomal types was detected and may have reflected variation of Rhizobium strains in the different geographical locations from which the strains originated. An investigation of pea strains with antibodies prepared against fava bean strains and restriction fragment length polymorphism analyses, targeting DNA regions coding for rRNA and nodulation, indicated that Rhizobium strains from V. faba nodules were distinguishable from those from Pisum sativum, V. villosa, and Trifolium spp.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference24 articles.

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2. Bottomley P. J. 1992. Ecology of Bradyrhizobium and Rhizobium p. 293-347. In G. Stacey H. J. Evans and R. H. Burris (ed.) Biological nitrogen fixation. Chapman and Hall New York.

3. Diversity within serogroups of Rhizobium leguminosarum biovar viciae in the Palouse region of Eastern Washington as indicated by plasmid profile, intrinsic antibiotic resistance, and topography;Brockman F. J.;Appl. Environ. Microbiol.,1989

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