Affiliation:
1. Centro Nacional de Biotecnología, CSIC, Madrid, Spain
Abstract
ABSTRACT
Stenotrophomonas maltophilia
is an opportunistic pathogen with increasing prevalence, which is able to cause infections in immunocompromised patients or in those with a previous pathology. The treatment of the infections caused by this bacterium is often complicated due to the several intrinsic antibiotic resistance mechanisms that it presents. Multidrug efflux pumps are among the best-studied mechanisms of
S. maltophilia
antibiotic resistance. Some of these efflux pumps have a basal expression level but, in general, their expression is often low and only reaches high levels when the local regulator is mutated or bacteria are in the presence of an effector. In the current work, we have developed a yellow fluorescent protein (YFP)-based sensor with the aim to identify effectors able to trigger the expression of SmeVWX, an efflux pump that confers resistance to quinolones, chloramphenicol, and tetracycline when it is expressed at high levels. With this purpose in mind, we tested a variety of different compounds and analyzed the fluorescence signal given by the expression of YFP under the control of the
smeVWX
promoter. Among the tested compounds, vitamin K
3
, which is a compound belonging to the 2-methyl-1,4-naphthoquinone family, is produced by plants in defense against infection, and has increasing importance in human therapy, was able to induce the expression of the SmeVWX efflux pump. In addition, a decrease in the susceptibility of
S. maltophilia
to ofloxacin and chloramphenicol was observed in the presence of vitamin K
3
, in both wild-type and
smeW
-deficient strains.
Funder
MINECO | Instituto de Salud Carlos III
Ministerio de Economía y Competitividad
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Cited by
20 articles.
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