Performance Characteristics of Updated INNO-LiPA Assays for Molecular Typing of Human Leukocyte Antigen A (HLA-A), HLA-B, and HLA-DQB1 Alleles-Reference-Cited by-同舟云学术

Performance Characteristics of Updated INNO-LiPA Assays for Molecular Typing of Human Leukocyte Antigen A (HLA-A), HLA-B, and HLA-DQB1 Alleles

Published:2004-03 Issue:2 Volume:11 Page:430-432
ISSN:1556-6811
Container-title:Clinical and Vaccine Immunology
language:en
Short-container-title:Clin Vaccine Immunol

Author:

De Vreese Karen¹²³⁴⁵,Barylski Rachel¹²³⁴⁵,Pughe Fiona¹²³⁴⁵,Bläser Miriam¹²³⁴⁵,Evans Colin¹²³⁴⁵,Norton John¹²³⁴⁵,Semana Gilbert¹²³⁴⁵,Holman Richard¹²³⁴⁵,Loiseau Pascale¹²³⁴⁵,Masson Dominique¹²³⁴⁵,Gielis Mieke¹²³⁴⁵,De Brauwer Annelies¹²³⁴⁵,De Canck Ilse¹²³⁴⁵,Verpooten Gonda¹²³⁴⁵,Hulstaert Frank¹²³⁴⁵

Affiliation:

1. Innogenetics N.V., B-9052 Ghent

2. Antwerp Blood Transfusion Center, Edegem, Belgium

3. Abbott-Murex Ltd., Dartford, Kent

4. The Anthony Nolan Trust, Royal Free Hospital, London, United Kingdom

5. Etablissement de Transfusion Sanguine, Rennes

Abstract

ABSTRACT We carried out a multicenter performance evaluation of three new DNA-based human leukocyte antigen (HLA) typing assays: INNO-LiPA HLA-A Update, INNO-LiPA HLA-B Update, and INNO-LiPA HLA-DQB1 Update. After optimization, the accuracy rates were all 100%, and the final observed resolutions were 99.4, 92.4, and 85.6%, respectively. These rapid and easy-to-perform assays yielded results fully concordant with other DNA-based tissue typing tests.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CDLI.11.2.430-432.2004

Reference2 articles.

1. Marsh, S. G., et al. 2001. Nomenclature for factors of the HLA system, 2000. Eur. J. Immunogenet.28:377-424.

2. Williams, T. M. 2001. Human leukocyte antigen gene polymorphism and the histocompatibility laboratory. J. Mol. Diagn.3:98-104.

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