Affiliation:
1. Institute of Immunology
2. Department of Gastroenterology
3. Department of Pathology, Otto-von-Guericke University Magdeburg
4. Institute of Medical Technology Magdeburg (IMTM) GmbH, 39120 Magdeburg, Germany
Abstract
ABSTRACT
Helicobacter pylori
is the key pathogen for gastroduodenal diseases. The clinical outcome of
H. pylori
infection is influenced by the presence of strain-specific virulence factors that are usually detected by the presence of specific anti-
H. pylori
antibodies in serum. Apart from the detection of these antibodies by enzyme-linked immunosorbent assay (ELISA), it is desirable to obtain additional information concerning the presence of certain virulence factors of
H. pylori
that are currently detected by immunoblot analysis. At present, the immunodiagnosis of an
H. pylori
infection includes two separate methods: ELISA and immunoblot analysis. Here, we report the development and evaluation of a new rapid flow microparticle immunofluorescence assay (FMIA) for detection of anti-
H. pylori
antibodies in human serum. The assay allows rapid qualitative and quantitative detection of anti-
H. pylori
antibodies by using crude antigen preparations as well as single recombinant antigens (urease A, urease B, CagA, and alkylhydroxy peroxide reductase) in the same sample with one measurement, and thus it combines the advantages of enzyme immunoassay and Western blot analysis. Seventy-five patient samples were analyzed by FMIA, ELISA, and Western blotting with respect to their immunoreactivity against crude
H. pylori
extracts and individual
H. pylori
antigens. Statistical analyses revealed an overall similarity of more than 90% among the results for FMIA, ELISA, and Western blot. Therefore, we conclude that FMIA is a powerful and time- and cost-saving assay system for the detection of antimicrobial antibodies, with higher sensitivity and a larger measurement range than ELISA.
Publisher
American Society for Microbiology
Subject
Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy
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