Affiliation:
1. Unité des Rickettsies, CNRS UMR 6020, Faculté de Médecine, Marseille, France
Abstract
ABSTRACT
Seven species-specific monoclonal antibodies (MAbs) were produced against
Encephalitozoon cuniculi
and characterized. The MAbs were immunoglobulin G, and when used for indirect microimmunofluorescence microscopy and Western immunoblot assays, they detected
E. cuniculi
originating from clinical samples. They did not cross-react with other
Encephalitozoon
species (
E. intestinalis
and
E. hellem
) or with a collection of gram-negative bacteria, yeast, and other parasites. The MAbs reacted primarily with 121-, 56-, 45-, 43-, and 41-kDa protein epitopes of
E. cuniculi.
These epitopes were demonstrated to be
E. cuniculi
species specific by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. We developed MAbs to strains of
E. cuniculi
that can be used successfully to distinguish
E. cuniculi
from other microsporidial species in cultures established from clinical specimens. These MAbs may provide a specific, simple, rapid, and low-cost tool for the identification and diagnosis of infections due to microsporidia.
Publisher
American Society for Microbiology
Subject
Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy
Reference35 articles.
1. Anwar-Bruni, D. M., S. E. Hogan., and D. A. Schwartz. 1996. Atovaquone is effective treatment for the symptom of gastrointestinal microsporidiosis in HIV-1-infected patients. AIDS10:619-624.
2. Bigliardi, E., M. G. Selmi, P. Lupetti, S. Corona, S. Gatti, M. Scaglia, and L. Sacchi. 1996. Microsporidian spore wall: ultrastructural findings on Encephalitozoon hellem exospore. J. Eukaryot. Microbiol.43:181-186.
3. Bryan, R. T., R. Weber, and D. A. Schwartz. 1997. Microsporidiosis in persons without HIV. Clin. Infect. Dis.24:534-535.
4. Croppo, G. P., G. S. Visvesvara, G. J. Leitch, S. Wallace, and D. A. Schwartz. 1998. Identification of the microsporidian Encephalitozoon hellem using immunoglobulin G monoclonal antibodies. Arch. Pathol. Lab. Med.122:182-186.
5. Didier, E. S. 1998. Microsporidiosis. Clin. Infect. Dis.27:1-7.
Cited by
16 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献