Comparison of Immunofluorescence, Western Blotting, and Cross-Adsorption Assays for Diagnosis of African Tick Bite Fever-Reference-Cited by-同舟云学术

Comparison of Immunofluorescence, Western Blotting, and Cross-Adsorption Assays for Diagnosis of African Tick Bite Fever

Published:2004-07 Issue:4 Volume:11 Page:786-788
ISSN:1556-6811
Container-title:Clinical and Vaccine Immunology
language:en
Short-container-title:Clin Vaccine Immunol

Author:

Jensenius Mogens¹²³⁴⁵,Fournier Pierre-Edouard¹²³⁴⁵,Vene Sirkka¹²³⁴⁵,Ringertz Signe Holta¹²³⁴⁵,Myrvang Bjørn¹²³⁴⁵,Raoult Didier¹²³⁴⁵

Affiliation:

1. Departments of Internal Medicine

2. Bacteriology, Aker University Hospital

3. Department of Infectious Diseases, Ullevål University Hospital, Oslo, Norway

4. Unité des Rickettsies, Université de la Méditerranée, Marseille, France

5. Swedish Institute for Infectious Disease Control, Solna, Sweden

Abstract

ABSTRACT In testing paired serum samples from 40 consecutive cases of African tick bite fever, we detected diagnostic antibodies against spotted fever group rickettsiae in 45% of the patients by immunofluorescence assay (IFA) and in 100% of the patients by Western blotting (WB) ( P < 0.01). A specific diagnosis of Rickettsia africae infection could be established in 15% of the patients by IFA and in 73% of the patients by a combination of WB and cross-adsorption assays ( P < 0.01).

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CDLI.11.4.786-788.2004

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