Affiliation:
1. Department of Virology and Epidemiology, Baylor College of Medicine, Houston, Texas 77025
2. Department of Microbiology, Medical College of Ohio at Toledo, Toledo, Ohio 43614
Abstract
Hepatitis B antigen of the D (a+, d+, y−) subtype was purified from plasma of apparently healthy persons and from hepatitis patients. The original samples contained 20- and 42-nm particles and tubular forms (20-nm diameter). Ultracentrifugation during the purification procedure yielded pellets which were then treated at
p
H 2.4. Both the large, 42-nm Dane particles and the tubular forms were lost during the acid treatment of the pelleted particles, yielding a preparation containing a mixture of particles approximately 20 and 25 nm in diameter. This difference in size was substantiated in that two distinct molecular weights were calculated from high-speed equilibrium data, 3.6 × 10
6
and 4.5 × 10
6
. Further heterogeneity was observed in that hepatitis B antigenic activity was present in purified particles with an isoelectric
p
H of 4.0 and also in those with a
p
H of 4.4. No significant differences were observed in the gross amino acid composition of purified antigen obtained from plasma of three different persons.
125
I-labeled, purified antigen was found to contain six distinct polypeptides with molecular weights ranging from 10,000 to 39,000.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Cited by
73 articles.
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