Identification of Key Genes during Ca 2+ -Induced Genetic Transformation in Escherichia coli by Combining Multi-Omics and Gene Knockout Techniques

Abstract

Using transcriptome and proteome techniques and association analysis, we identified several key genes involved in the formation of Ca 2+ -mediated E. coli DH5α competent cells. We used Red homologous recombination technology to construct three single-gene deletion strains and found that the transformation efficiencies of yiaW , ygiZ , and osmB deletion strains for different-size plasmids were significantly increased.