Susceptibility Testing of Anaerobic Bacteria: Evaluation of the Redesigned (Version 96) bioMérieux ATB ANA Device

Author:

Dubreuil L.1,Houcke I.1,Singer E.1

Affiliation:

1. Faculté de Pharmacie, Lille, France

Abstract

ABSTRACT We compared the susceptibility results for 200 clinical anaerobes with nine antibiotics obtained by using a new ATB ANA (bioMérieux) device against those obtained by the National Committee for Clinical Laboratory Standards (NCCLS) standard agar dilution method. For better evaluation of the device, we added some resistant Bacteroides fragilis group strains from our own collection: 3, 6, and 12 strains that were resistant to imipenem, ticarcillin plus clavulanic acid, and co-amoxiclav, respectively, and 2 other strains with decreased susceptibility to metronidazole. For some strains that did not grow on ATB S medium, tests were performed by using West-Wilkins medium supplemented with 1.5% agar. The new ATB ANA device made clinical categorization of the investigated strains possible, according to French (Committee of the Antibiogram of the French Society of Microbiology) or U.S. (NCCLS) breakpoints, with the following respective results: category agreement, 94.3 and 94.9%; minor errors, 4.8 and 3.8%; major errors, 0.4 and 0.8%; and very major errors 4.6 and 4.2%. The ATB ANA device was able to detect low-level metronidazole-resistant B. fragilis strains according to the French breakpoints but not the NCCLS ones. For B. fragilis and β-lactamase-positive Prevotella strains, the clustering effect of amoxicillin MICs around the French breakpoints led to more frequent minor errors. ATB ANA is a very convenient method to determine the antibiotic susceptibilities of anaerobes. Results obtained by ATB ANA correlated well with those obtained by the reference method.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference22 articles.

1. Communiqué 1996 du Comité de l’antibiogramme de la Société Francaise de Microbiologie. Valeurs critiques pour l’antibiogramme;Acar J.;Pathol. Biol.,1996

2. Characterization of beta-lactamases from non-Bacteroides fragilis group Bacteroides spp. belonging to seven species and their role in beta-lactam resistance

3. In vitro activity of three carbapenems biapenem, imipenem and meropenem and some other antibiotics against anaerobic bacteria;Bland S.;Pathol. Biol.,1995

4. Survey of the susceptibility patterns of Bacteroides fragilis group in France from 1977 to 1992;Dubreuil L.;Eur. J. Clin. Microbiol.,1992

5. In vitro evaluation of activities of nitazoxanide and tizoxanide against anaerobes and aerobic organisms

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