Affiliation:
1. Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik, D-7000 Stuttgart 80, Technische Universität Hamburg-Harburg, D-2100 Hamburg 90, 2 and Universität Stuttgart, D-7000 Stuttgart 1, 3 Federal Republic of Germany
Abstract
Strain DM1, a
Mycobacterium
sp. that utilizes 2,6-xylenol, 2,3,6-trimethylphenol, and
o
-cresol as sources of carbon and energy, was isolated. Intact cells of
Mycobacterium
strain DM1 grown with 2,6-xylenol cooxidized 2,4,6-trimethylphenol to 2,4,6-trimethylresorcinol. 4-Chloro-3,5-dimethylphenol prevents 2,6-xylenol from being totally degraded; it was quantitatively converted to 2,6-dimethylhydroquinone by resting cells. 2,6-Dimethylhydroquinone, citraconate, and an unidentified metabolite were detected as products of 2,6-xylenol oxidation in cells that were partially inactivated by EDTA. Under oxygen limitation, 2,6-dimethylhy-droquinone, citraconate, and an unidentified metabolite were released during 2,6-xylenol turnover by resting cells. Cell extracts of 2,6-xylenol-grown cells contained a 2,6-dimethylhydroquinone-converting enzyme. When supplemented with NADH, cell extracts catalyzed the reduction of 2,6-dimethyl-3-hydroxyquinone to 2,6-dimethyl-3-hydroxyhydroquinone. Since a citraconase was also demonstrated in cell extracts, a new metabolic pathway with 2,6-dimethyl-3-hydroxyhydroquinone as the ring fission substrate is proposed.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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