Comparison of Pulsed-Field Gel Electrophoresis and Coagulase Gene Restriction Profile Analysis Techniques in the Molecular Typing of Staphylococcus aureus

Author:

Chiou Chien-Shun1,Wei Hsiao-Lun1,Yang Li-Chu1

Affiliation:

1. The Third Branch Office, Center for Disease Control, Department of Health, Taichung City, Taiwan

Abstract

ABSTRACT Pulsed-field gel electrophoresis (PFGE) and coagulase gene restriction profile (CRP) analysis techniques were used to analyze 71 Staphylococcus aureus isolates recovered from nine food-borne disease outbreaks. Twenty-two PFGE profiles and 11 CRPs were identified, with discrimination indices of 0.86 and 0.72, respectively. In addition, the variable regions of the coagulase genes of 39 isolates were sequenced and showed extensive identity, indicating that this is not an efficient alternative for the molecular typing of S. aureus .

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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