Attachment and Structural Features of Flagella of Certain Bacilli

Abstract

Abram, Dinah (Purdue University, Lafayette, Ind.), A. E. Vatter, and Henry Koffler . Attachment and structural features of flagella of certain bacilli. J. Bacteriol. 91: 2045–2068. 1966.—The attachment of flagella to cells of various mesophilic and thermophilic strains of Bacillus was studied electron microscopically. Studies of ghost cells and membrane fragments indicate that flagella are connected to the cytoplasmic membrane. Flagella removed from cells mechanically, during autolysis, or by phage lysis, have attached to the base of their proximal hooks material that is heterogeneous in character. In part, this material consists of cytoplasmic membrane; its varied shape appears to be caused by the folding of the membrane around the proximal end of the flagellum at the site of attachment. It is uncertain whether this material represents a real structure or an artifact. Highresolution microscopy reveals differences in the fine structure of intact flagella of the various strains studied. The proximal hook and the flagellar filament are distinct in morphology and fine structure. A specialized structure is associated with the hook of flagella of B. brevis and B. circulans . The filament of flagella of B. stearothermophilus 2184 has two regions that show marked differences in the manner in which the subunits appear to be organized. No correlation was found between the site of origin of flagella and the location of reduced tellurite when the reduction of potassium tellurite was used to indicate the loci of enzymatic respiratory activities.