Chromosomal Location of the Structural Gene for Glycerol Kinase in Escherichia coli

Abstract

Cozzarelli , N. R. (Harvard Medical School, Boston, Mass.), and E. C. C. Lin . Chromosomal location of the structural gene for glycerol kinase in Escherichia coli . J. Bacteriol. 91: 1763–1766. 1966.—A glycerol kinase mutant site has been mapped by transduction and sexual conjugation. Three-factor crosses with the two procedures yielded the following gene order: arginine-1–methionine-1–glycerol kinase–isoleucine, valine-16. An additional 13 independent glycerol kinase mutant sites mapped in the same region. Since some of the mutants were able to produce a protein serologically indistinguishable from the wild-type enzyme, it is concluded that the region mapped represents the structural gene for the kinase.