Performance of the OraQuick Rapid Antibody Test for Diagnosis of Human Immunodeficiency Virus Type 1 Infection in Patients with Various Levels of Exposure to Highly Active Antiretroviral Therapy

Author:

O'Connell Robert J.1,Merritt Teresa M.1,Malia Jennifer A.2,VanCott Thomas C.3,Dolan Matthew J.1,Zahwa Hassan2,Bradley William P.3,Branson Bernard M.4,Michael Nelson L.2,De Witt Caroline C.1

Affiliation:

1. Wilford Hall Medical Center, Lackland Air Force Base, Texas 78236

2. Division of Retrovirology, Walter Reed Army Institute for Research

3. Henry M. Jackson Foundation, Rockville, Maryland 20850

4. National Center for HIV, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia 30333

Abstract

ABSTRACT With oral mucosal transudate and serum samples from 101 human immunodeficiency virus type 1 (HIV-1)-infected subjects and 100 HIV-1-negative volunteers, the OraQuick HIV-1 test demonstrated 100% specificity and 96% sensitivity. Four false-negative subjects, who were characterized by early initiation of effective antiretroviral therapy, demonstrated waning serum anti-gp41 titers and Western blot band intensities.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference12 articles.

1. Bakari, J. P., S. McKenna, A. Myrick, K. Mwinga, G. J. Bhat, and S. Allen. 2000. Rapid voluntary testing and counseling for HIV. Acceptability and feasibility in Zambian antenatal care clinics. Ann. N. Y. Acad. Sci.918:64-76.

2. Centers for Disease Control and Prevention. 2001. Routinely recommended HIV testing at an urgent-care clinic, Atlanta, Georgia, 2000. Morb. Mortal. Wkly. Rep.50:538-541.

3. Centers for Disease Control and Prevention. 1998. Update: HIV counseling and testing using rapid tests—United States, 1995. Morb. Mortal. Wkly. Rep.47:211-215.

4. Analysis of Genetic Variability within the Immunodominant Epitopes of Envelope gp41 from Human Immunodeficiency Virus Type 1 (HIV-1) Group M and Its Impact on HIV-1 Antibody Detection

5. Giles, R. E., K. R. Perry, and J. V. Parry. 1999. Simple/rapid test devices for anti-HIV screening: do they come up to the mark? J. Med. Virol.59:104-109.

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