Genetic Differentiation of Escherichia coli O157:H7 Clades Associated with Human Disease by Real-Time PCR-Reference-Cited by-同舟云学术

Genetic Differentiation of Escherichia coli O157:H7 Clades Associated with Human Disease by Real-Time PCR

Published:2008-06 Issue:6 Volume:46 Page:2070-2073
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Riordan James T.¹,Viswanath Sandeep B.¹,Manning Shannon D.¹,Whittam Thomas S.¹

Affiliation:

1. Microbial Evolution Laboratory, National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824

Abstract

ABSTRACT The rapid and accurate identification of Escherichia coli O157:H7 strains is central to reducing the impact of outbreaks. A real-time PCR-based approach to differentiating major outbreak lineages of O157 with novel single-nucleotide polymorphisms is described. The utility of this method is in detection of hypervirulent strains in cases of clinical disease.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00203-08

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