Comparative Evaluation of AmpliVue HSV 1+2 Assay with ELVIS Culture for Detecting Herpes Simplex Virus 1 (HSV-1) and HSV-2 in Clinical Specimens-Reference-Cited by-同舟云学术

Comparative Evaluation of AmpliVue HSV 1+2 Assay with ELVIS Culture for Detecting Herpes Simplex Virus 1 (HSV-1) and HSV-2 in Clinical Specimens

Published:2015-12 Issue:12 Volume:53 Page:3922-3925
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Granato Paul A.¹²^ORCID,Alkins Brenda R.¹,Yen-Lieberman Belinda³,Greene Wallace H.⁴,Connolly Jessica⁵,Buchan Blake W.⁵^ORCID,Ledeboer Nathan A.⁵

Affiliation:

1. Laboratory Alliance of Central New York, Liverpool, New York, USA

2. SUNY Upstate Medical University, Syracuse, New York, USA

3. Cleveland Clinic, Cleveland, Ohio, USA

4. Penn State Hershey Medical Center, Hershey, Pennsylvania, USA

5. The Medical College of Wisconsin, Milwaukee, Wisconsin, USA

Abstract

ABSTRACT The AmpliVue HSV 1+2 assay was compared to the ELVIS HSV ID and D 3 Typing Culture System for the qualitative detection and differentiation of herpes simplex virus 1 (HSV-1) and HSV-2 DNA in 1,351 cutaneous and mucocutaneous specimens. Compared to ELVIS, AmpliVue had sensitivities of 95.7 and 97.6% for detecting HSV-1 and HSV-2, respectively. Following arbitration of discordant results by an independent molecular method, the AmpliVue assay had a resolved sensitivity and specificity of 99.2 and 99.7%, respectively, for both HSV-1 and HSV-2, whereas ELVIS had a resolved sensitivity of 87.1% for HSV-1 and 84.5% for HSV-2.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01905-15

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