Primary Swine Respiratory Epithelial Cell Lines for the Efficient Isolation and Propagation of Influenza A Viruses-Reference-Cited by-同舟云学术

Primary Swine Respiratory Epithelial Cell Lines for the Efficient Isolation and Propagation of Influenza A Viruses

Published:2020-11-23 Issue:24 Volume:94 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Meliopoulos Victoria¹^ORCID,Cherry Sean¹,Wohlgemuth Nicholas¹,Honce Rebekah¹²,Barnard Karen³^ORCID,Gauger Phillip⁴^ORCID,Davis Todd⁵,Shult Peter⁶,Parrish Colin³^ORCID,Schultz-Cherry Stacey¹^ORCID

Affiliation:

1. Department of Infectious Diseases, St. Jude Children’s Research Hospital, Memphis, Tennessee, USA

2. Integrated Program in Biomedical Sciences, College of Graduate Health Sciences, University of Tennessee Health Science Center, Memphis, Tennessee, USA

3. Department of Microbiology and Immunology, Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA

4. Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA

5. Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control, Atlanta, Georgia, USA

6. Wisconsin State Laboratory of Hygiene, University of Wisconsin-Madison, Madison, Wisconsin, USA

Abstract

Robust in vitro culture systems for influenza virus are critically needed. MDCK cells, the most widely used cell line for influenza isolation and propagation, do not adequately model the respiratory tract. Therefore, many clinical isolates, both animal and human, are unable to be isolated and characterized, limiting our understanding of currently circulating influenza viruses. We have developed immortalized swine respiratory epithelial cells that retain the ability to differentiate and can support influenza replication and isolation. These cell lines can be used as additional tools to enhance influenza research and vaccine development.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

American Lebanese Syrian Associated Charities

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.01091-20

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