Affiliation:
1. Department of Pathology, University of Texas Medical Branch, Galveston 77550.
Abstract
One thousand four hundred forty-seven clinical and environmental isolates of molds, yeasts, aerobic actinomycetes, and algae belonging to 164 genera (382 taxa) maintained on potato dextrose agar at -70 degrees C for periods ranging from 6 months to 13 years were subcultured and then incubated at 25 degrees C to determine their viabilities. Thirty-three isolates, Alternaria alternata (n = 1), Apophysomyces elegans (n = 1), Bipolaris spicifera (n = 1), Blastomyces dermatitidis (n = 4), Cokeromyces recurvatus (n = 1), Coremiella cubispora (n = 1), Cryptococcus ater (n = 1), Curvularia sp. (n = 1), Exserohilum monoceras (n = 1), Exserohilum pedicillatum (n = 1), Exserohilum rostratum (n = 1), Filobasidium floriforme (n = 1), Madurella mycetomatis (n = 1), Oedocephalum spp. (n = 2), Penicillium marneffei (n = 1), Pseudomicrodochium spp. (n = 4), Saksenaea vasiformis (n = 1), Sporothrix sp. (n = 1), and Mycelia Sterilia (n = 8), did not grow after repeated attempts at subculturing. Neither time in storage nor taxonomic classification was associated with a lack of viability. Storage at low temperature for either short or long periods of time is an excellent method for maintaining most medically important fungi.
Publisher
American Society for Microbiology
Reference8 articles.
1. Soil as a storage medium for fungi;Bakerspiegel A.;Mycologia,1953
2. Application of the mineral oil conservation method for maintaining collections of fungus cultures;Buell C. B.;Am. J. Bot.,1947
3. Viability of mold cultures stored at -20°C;Carmichael J. W.;Mycologia,1962
4. The viability of some pathogenic fungi in sterile distilled water;Castellani A.;J. Trop. Med. Hyg.,1939
5. Investigation of ultra low temperature for fungal cultures. III. Viability and growth rate of mycelial cultures following cryogenic storage;Hwang S. H.;Mycologia,1976
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