Affiliation:
1. Institut de Bactériologie de la Faculté de Médecine, Strasbourg, France.
Abstract
Pulsed-field gel electrophoresis (PFGE) after SmaI restriction of DNA from 239 methicillin-resistant Staphylococcus aureus isolates (from 142 patients) produced 26 different fingerprints. The deduced chromosome sizes ranged from 2,200 to 3,100 kb (+/- 100 kb). A total of 81 isolates taken from 65 patients were then typed by PFGE and ribotyping with ClaI, EcoRI, and HindIII. Ribotypes were less discriminating than PFGE. Ribotyping did not discriminate isolates from a given PFGE fingerprint into different subsets. PFGE may be a more effective epidemiological tool than ribotyping for the typing of methicillin-resistant S. aureus strains.
Publisher
American Society for Microbiology
Reference22 articles.
1. Communique 1990 du Comite de l'Antibiogramme de la Soci 6te Francaise de;Acar J.;Microbiologie. Pathol. Biol.,1990
2. Use of low-frequency-cleavage restriction endonucleases for DNA analysis in epidemiological investigations of nosocomial bacterial infections;Allardet-Servent A.;J. Clin. Microbiol.,1989
3. Barry A. L. and C. Thornsberry. 1991. Susceptibility tests: diffusion test procedures p. 1117-1125. In A. Balows W. J. Hausler Jr. K. L. Herrmann H. D. Isenberg and H. J. Shadomy (ed.) Manual of clinical microbiology 5th ed. American Society for Microbiology Washington D.C.
4. Phage-typing of staphylococci;Blair J. E.;Bull. W. H. O.,1961
5. Staff carriage of epidemic methicillin-resistant Staphylococcus aureus;Cookson B.;J. Clin. Microbiol.,1989
Cited by
231 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献