Affiliation:
1. Department of Agricultural Chemistry, Kyoto University, Kitashirakawa, Oiwake-cho, Sakyo-ku, Kyoto 606, Japan
Abstract
Nitrile hydratase, which occurs abundantly in cells of
Rhodococcus rhodochrous
J1 isolated from soil samples, catalyzes the hydration of 3-cyanopyridine to nicotinamide. By using resting cells, the reaction conditions for nicotinamide production were optimized. Under the optimum conditions, 100% of the added 12 M 3-cyanopyridine was converted to nicotinamide without the formation of nicotinic acid, and the highest yield achieved was 1,465 g of nicotinamide per liter of reaction mixture containing resting cells (1.48 g as dry cell weight) in 9 h. The nicotinamide produced was crystallized and then identified physicochemically. The further conversion of the nicotinamide to nicotinic acid was due to the low activity of nicotinamide as a substrate for the amidase(s) present in this organism.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference9 articles.
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5. FIG. 3. Time course of the enzymatic hydration of 3-cyanopyri
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