Influence of Molecular Size and Ligninase Pretreatment on Degradation of Lignins by Xanthomonas sp. Strain 99-Reference-Cited by-同舟云学术

Influence of Molecular Size and Ligninase Pretreatment on Degradation of Lignins by Xanthomonas sp. Strain 99

Published:1987-09 Issue:9 Volume:53 Page:2242-2246
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Kern Hartmut W.¹,Kirk T. Kent¹

Affiliation:

1. Institut für Biotechnologie, Kernforschungsanlage Jülich, D-5170 Jülich, Federal Republic of Germany, and Forest Products Laboratory, Forest Service, U.S. Department of Agriculture, Madison, Wisconsin 53705-23982

Abstract

The purpose of this study was to examine the relationship between the molecular size of lignin in several preparations and extent of degradation (mineralization) by Xanthomonas sp. strain 99. The influence of ligninase pretreatment was also examined. Five synthetic lignins and one 14 C-methylated spruce lignin were used. The extent of mineralization to 14 CO 2 was greatest for the samples containing the most low-molecular-weight material, and the low-molecular-weight portions were preferentially (or perhaps solely) degraded. Pretreatment of the five synthetic lignins with crude ligninase increased their molecular size and decreased their degradability by the xanthomonad. Pretreatment of the methylated spruce lignin with crude ligninase caused both polymerization and depolymerization but resulted in a net decrease in bacterial degradability. Our results suggest that the xanthomonad can degrade lignins only up to a molecular weight of 600 to 1,000.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.53.9.2242-2246.1987

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