Simple and Reliable Method To Precipitate Proteins from Bacterial Culture Supernatant-Reference-Cited by-同舟云学术

Simple and Reliable Method To Precipitate Proteins from Bacterial Culture Supernatant

Published:2004-01 Issue:1 Volume:70 Page:610-612
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Caldwell Randolph B.¹,Lattemann Claus T.¹

Affiliation:

1. CREATOGEN AG, D-86156 Augsburg, Germany

Abstract

ABSTRACT A simple and reliable method for precipitating protein from bacterial culture supernatants based on a pyrogallol red-molybdate-methanol (PRMM) protocol has been developed and applied for the analysis of proteins secreted by a bacterial type III secretion system. PRMM-based precipitation has been shown to be more efficient and robust than are conventional protocols.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.70.1.610-612.2004

Reference16 articles.

1. Aguilar, R. M., J. J. Bustamante, P. G. Hernandez, A. O. Martinez, and L. S. Haro. 1999. Precipitation of dilute chromatographic samples (ng/ml) containing interfering substances for SDS-PAGE. Anal. Biochem.267:344-350.

2. Beuzon, C. R., G. Banks, J. Deiwick, M. Hensel, and D. W. Holden. 1999. pH-dependent secretion of SseB, a product of the SPI-2 type III secretion system of Salmonella typhimurium. Mol. Microbiol.33:806-816.

3. Deiwick, J., T. Nikolaus, S. Erdogan, and M. Hensel. 1999. Environmental regulation of Salmonella pathogenicity island 2 gene expression. Mol. Microbiol.31:1759-1773.

4. Hensel, M., J. E. Shea, S. R. Waterman, R. Mundy, T. Nikolaus, G. Banks, A. Vazquez-Torres, C. Gleeson, F. C. Fang, and D. W. Holden. 1998. Genes encoding putative effector proteins of the type III secretion system of Salmonella pathogenicity island 2 are required for bacterial virulence and proliferation in macrophages. Mol. Microbiol.30:163-174.

5. Type III Protein Secretion Systems in Bacterial Pathogens of Animals and Plants

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