Affiliation:
1. Department of Entomology, Kansas State University, Manhattan, Kansas
Abstract
ABSTRACT
The ecology of
Escherichia coli
O157:H7 is not well understood. The aims of this study were to determine the prevalence of and characterize
E. coli
O157:H7 associated with houseflies (HF).
Musca domestica
L. HF (
n
= 3,440) were collected from two sites on a cattle farm over a 4-month period and processed individually for
E. coli
O157:H7 isolation and quantification. The prevalence of
E. coli
O157:H7 was 2.9 and 1.4% in HF collected from feed bunks and a cattle feed storage shed, respectively.
E. coli
O157:H7 counts ranged from 3.0 × 10
1
to 1.5 × 10
5
CFU among the positive HF. PCR analysis of the
E. coli
O157:H7 isolates revealed that 90.4, 99.2, 99.2, and 100% of them (
n
= 125) possessed the
stx1
,
stx2
,
eaeA
, and
fliC
genes, respectively. Large populations of HF on cattle farms may play a role in the dissemination of
E. coli
O157:H7 among animals and to the surrounding environment.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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