Affiliation:
1. VTT Biotechnology, Espoo, Finland
2. Genencor International, Inc., Palo Alto, California
Abstract
ABSTRACT
Two genes involved in protein secretion, encoding the Rab protein YPT1/YPTA and the general fusion factor NSFI/NSFA, were characterized from two filamentous fungi,
Trichoderma reesei
and
Aspergillus niger
var.
awamori
. The isolated genes showed a high level of conservation with their
Saccharomyces cerevisiae
and mammalian counterparts, and
T. reesei ypt1
was shown to complement yeast Ypt1p depletion. The transcriptional regulation of the
T. reesei ypt1
,
nsf1
, and
sar1
genes, involved in protein trafficking, was studied with mycelia treated with the folding inhibitor dithiothreitol (DTT) and with brefeldin A, which inhibits membrane traffic between the endoplasmic reticulum and Golgi complex. The well-known inducer of the yeast and
T. reesei
unfolded protein response (UPR), DTT, induced the
nsf1
gene and the protein disulfide isomerase gene,
pdi1
, in both of the experiments, and
sar1
mRNA increased in only one experiment under strong UPR induction. The
ypt1
mRNA did not show a clear increase during DTT treatment. Brefeldin A strongly induced
pdi1
and all of the intracellular trafficking genes studied. These results suggest the possibility that the whole secretory pathway of
T. reesei
could be induced at the transcriptional level by stress responses caused by protein accumulation in the secretory pathway.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
30 articles.
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