Reevaluation and Reduction of a PCR Bias Caused by Reannealing of Templates-Reference-Cited by-同舟云学术

Reevaluation and Reduction of a PCR Bias Caused by Reannealing of Templates

Published:2004-12 Issue:12 Volume:70 Page:7545-7549
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Kurata Shinya¹²³,Kanagawa Takahiro²³,Magariyama Yukio⁴,Takatsu Kyoko¹²,Yamada Kazutaka¹²,Yokomaku Toyokazu¹,Kamagata Yoichi²

Affiliation:

1. Kankyo Engineering Co., Ltd., Kisarazu

2. Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba

3. Department of Molecular Engineering, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama

4. Food Engineering Division, National Food Research Institute, Tsukuba, Ibaraki, Japan

Abstract

ABSTRACT We reevaluated the bias toward a 1:1 ratio of products in multitemplate PCR used in ecological studies and showed that the template reannealing at the annealing step would not cause the bias; however, the preferential homoduplex formation during temperature decrease from denaturation to annealing step would cause the bias.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.70.12.7545-7549.2004

Reference23 articles.

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