Mapping Loci for Surface Exclusion and Incompatibility on the F Factor of Escherichia coli K-12

Abstract

A series of Hfr deletion strains carrying deletions extending different distances into the integrated F factor have been used to map loci for surface exclusion ( traS ) and for incompatibility ( inc ) on the Escherichia coli K-12 sex factor F. traS mapped between traG and traD . It forms a part of the large operon, including all the known transfer genes except traJ , and is co-controlled with these. The product of traS is not required for formation of the F pilus. inc mapped between the φ R 11 locus and the origin of transfer; it is therefore one of the earliest loci transferred during conjugation.