Culture-Based Virus Isolation To Evaluate Potential Infectivity of Clinical Specimens Tested for COVID-19-Reference-Cited by-同舟云学术

Culture-Based Virus Isolation To Evaluate Potential Infectivity of Clinical Specimens Tested for COVID-19

Published:2020-07-23 Issue:8 Volume:58 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Huang Chung-Guei¹²³,Lee Kuo-Ming³^ORCID,Hsiao Mei-Jen¹,Yang Shu-Li¹,Huang Peng-Nien³⁴,Gong Yu-Nong¹³,Hsieh Tzu-Hsuan¹,Huang Po-Wei¹,Lin Ya-Jhu¹,Liu Yi-Chun¹,Tsao Kuo-Chien¹²³,Shih Shin-Ru¹²³⁵^ORCID

Affiliation:

1. Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan

2. Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, Taiwan

3. Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan, Taiwan

4. Division of Infectious Diseases, Department of Pediatrics, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan

5. Research Center for Chinese Herbal Medicine, Research Center for Food and Cosmetic Safety, and Graduate Institute of Health Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, Taiwan

Abstract

Real-time reverse transcription-PCR (RT-PCR) is currently the most sensitive method to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes coronavirus disease 2019 (COVID-19). However, the correlation between detectable viral RNA and culturable virus in clinical specimens remains unclear. Here, we performed virus culture for 60 specimens that were confirmed to be positive for SARS-CoV-2 RNA by real-time RT-PCR. The virus could be successfully isolated from 12 throat and nine nasopharyngeal swabs and two sputum specimens.

Funder

Taiwan Centers for Disease Control

Ministry of Science and Technology, Taiwan

Ministry of Education

Chang Gung Memorial Hospital

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01068-20

Reference27 articles.