Transformation of Azotobacter vinelandii with Plasmids RP4 (IncP-1 Group) and RSF1010 (IncQ Group)-Reference-Cited by-同舟云学术

Transformation of Azotobacter vinelandii with Plasmids RP4 (IncP-1 Group) and RSF1010 (IncQ Group)

Published:1981-06 Issue:3 Volume:146 Page:1154-1157
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

David Michel¹,Tronchet Maurice¹,Dénarié Jean¹

Affiliation:

1. Laboratoire de Génétique des Microorganismes, Institut National de la Recherche Agronomique, 78000 Versailles, France

Abstract

Multicopy plasmid RSF1010 and four of its in vitro-constructed derivatives were mobilized by the self-transmissible RP4 plasmid into Azotobacter vinelandii UW. Modifications of the Escherichia coli transformation procedure of Cohen et al. (Proc. Natl. Acad. Sci. U.S.A. 69: 2110–2114, 1972) allowed transformation of A. vinelandii strains UW and ATCC 12837 with purified RP4 or RSF1010 deoxyribonucleic acid.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.146.3.1154-1157.1981

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