Passive hemagglutination test for detection of antibodies to human immunodeficiency virus type 1 and comparison of the test with enzyme-linked immunosorbent assay and Western blot (immunoblot) analysis-Reference-Cited by-同舟云学术

Passive hemagglutination test for detection of antibodies to human immunodeficiency virus type 1 and comparison of the test with enzyme-linked immunosorbent assay and Western blot (immunoblot) analysis

Published:1989-01 Issue:1 Volume:27 Page:179-181
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Vasudevachari M B¹,Uffelman K W¹,Mast T C¹,Dewar R L¹,Natarajan V¹,Lane H C¹,Salzman N P¹

Affiliation:

1. Division of Molecular Virology and Immunology, Georgetown University School of Medicine, Washington, D.C. 20007.

Abstract

A passive hemagglutination test (PHA) was developed for detecting antibodies to human immunodeficiency virus type 1 (HIV-1) utilizing sheep erythrocytes cross-linked with purified envelope glycoprotein (gp160) of HIV-1. In an analysis of 216 human serum samples, 100% correlation was observed in 86 reactive and 124 nonreactive serum samples between PHA and commercial enzyme-linked immunosorbent assays and Western blot (immunoblot) analysis. Serum samples from gp160-immunized chimpanzees also reacted equally well in PHA. The test is simple, rapid, and inexpensive, thus providing an alternate, quick method of detecting HIV antibodies. These advantages and the thermal stability of the reagents that are used make this an attractive alternative for detecting prior exposure of individuals to HIV-1.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.27.1.179-181.1989

Reference24 articles.

1. Isolation of a Tlymphocyte retrovirus from a patient at risk for acquired immunodeficiency syndrome (AIDS);Barre-Sinoussi F.;Science,1983

2. Biberfeld G. U. Bredberg-Raden B. Bottiger P. Putkonen J. Blomberg P. Juto and G. Wadell. 1986. Blood donor sera with false-positive Western blot reactions to human immunodeficiency virus. Lancet ii:289-290.

3. Detection of HIV antigen and specific antibodies to HIV core and envelope proteins in sera of patients with HIV infection;Cao Y.;Blood,1987

4. Crofts J. N. W. J. Maskill D. S. Healy and I. D. Gust. 1987. Particle agglutination assay for anti-HIV. Lancet ii:797-798.

5. Lymphadenopathy associated virus infection of a blood donor-recipient pair with acquired immunodeficiency syndrome;Feorino P. M.;Science,1984

Cited by 7 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Evaluation and Correlation of Rabies Vaccine Potency Using the National Institute of Health, Rapid Focus Fluorescent Inhibition, and Passive Hemagglutination Tests;Viral Immunology;2022-03-01

2. Application of frozen and stored glutaraldehyde-fixed turkey red blood cells for hemagglutination and hemagglutination inhibition assays for the detection and identification of influenza viruses;Journal of Virological Methods;2021-03

3. Characterization of a discontinuous epitope of the human immunodeficiency virus (HIV) core protein p24 by epitope excision and differential chemical modification followed by mass spectrometric peptide mapping analysis;Protein Science;2008-12-31

4. Whole-Blood Agglutination Assay for On-Site Detection of Human Immunodeficiency Virus Infection;Journal of Clinical Microbiology;2003-07

5. Molecular screening on a compact disc;Organic & Biomolecular Chemistry;2003