Quality control guidelines for testing cefotetan in the reference agar dilution procedure for susceptibility testing of anaerobic bacteria

Author:

Zabransky R J1,Bobey D G1,Barry A L1,Allen S D1,Fuchs P C1,Gerlach E H1,Thornsberry C1,Sheikh W1,Jones R N1

Affiliation:

1. Department of Laboratory Medicine, Sinai Samaritan Medical Center, Milwaukee, Wisconsin 53201.

Abstract

Reference values for quality control of in vitro susceptibility tests with cefotetan against anaerobic bacteria were determined in two independent multilaboratory studies with the approved National Committee for Clinical Laboratory Standards agar dilution method and three control strains (Bacteroides fragilis ATCC 25285, Bacteroides thetaiotaomicron ATCC 29741, and Clostridium perfringens ATCC 13124). The results of the two studies were in agreement. The recommended MIC control limits for B. fragilis ATCC 25285 and B. thetaiotaomicron ATCC 29741 are 4.0 to 16 micrograms/ml and 32 to 128 micrograms/ml, respectively. MICs for C. perfringens ATCC 13124 were too variable to be useful for controlling tests with cefotetan.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference8 articles.

1. Criteria for cefotetan in vitro susceptibility testing including correlations with clinical and bacteriologic responses;Barry A. L.;Am. J. Surg.,1988

2. National Committee for Clinical Laboratory Standards. 1985. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M7-A. National Committee for Clinical Laboratory Standards Villanova Pa.

3. National Committee for Clinical Laboratory Standards. 1985. Referencesgar dilution procedure for antimicrobial susceptibility testing of anaerobic bacteria. Approved standard Ml 1-A. National Committee for Clinical Laboratory Standards Villanova Pa.

4. National Committee for Clinical Laboratory Standards. 1987. Development of in vitro susceptibility testing criteria and quality control parameters. Proposed guideline M23-P. National Committee for Clinical Laboratory Standards Villanova Pa.

5. National Committee for Clinical Laboratory Standards. 1987. Performance standards for antimicrobial susceptibility testing. Second informational supplement M100-S2. National Committee for Clinical Laboratory Standards Villanova Pa.

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