Participation of Valine 171 in α-Helix 5 of Bacillus thuringiensis Cry1Ab δ-Endotoxin in Translocation of Toxin into Lymantria dispar Midgut Membranes-Reference-Cited by-同舟云学术

Participation of Valine 171 in α-Helix 5 of Bacillus thuringiensis Cry1Ab δ-Endotoxin in Translocation of Toxin into Lymantria dispar Midgut Membranes

Published:2010-12 Issue:23 Volume:76 Page:7878-7880
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Alzate Oscar¹²,Osorio Cristina¹,Florez Alvaro M.³,Dean Donald H.¹

Affiliation:

1. Biochemistry Department, The Ohio State University, 484 W. 12th Avenue, Columbus, Ohio 43210

2. Escuela de Medicina, Universidad Pontificia Bolivariana, Medellín, Colombia

3. Laboratorio de Biología Molecular y Biotecnología, Facultad de Medicina, Universidad de Santander, Bucaramanga, Colombia

Abstract

ABSTRACT The Cry1Ab δ-endotoxin V171C mutant protein exhibits a 25-fold increase in toxicity against Lymantria dispar , which correlates with a faster rate of partitioning into the midgut membrane and slightly decreased protein stability. This is an insect-specific mechanism; similar results were not observed in Manduca sexta , another Cry1Ab δ-endotoxin-susceptible insect.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.01428-10

Reference12 articles.

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2. Alzate, O., C. F. Hemann, C. Osorio, R. Hille, and D. H. Dean. 2009. Ser170 of Bacillus thuringiensis Cry1Ab δ-endotoxin becomes anchored in a hydrophobic moiety upon insertion of this protein into Manduca sexta brush border membranes. BMC Biochem.10:25.

3. Alzate, O., T. You, M. Claybon, C. Osorio, A. Curtiss, and D. H. Dean. 2006. Effects of disulfide bridges in domain I of Bacillus thuringiensis Cry1Aa δ-endotoxin on ion-channel formation in biological membranes. Biochemistry45:13597-13605.

4. Production of Chymotrypsin-Resistant Bacillus thuringiensis Cry2Aa1 δ-Endotoxin by Protein Engineering

5. Effect of Insect Larval Midgut Proteases on the Activity of Bacillus thuringiensis Cry Toxins

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