Evaluation of the Amplex eazyplex Loop-Mediated Isothermal Amplification Assay for Rapid Diagnosis of Pneumocystis jirovecii Pneumonia-Reference-Cited by-同舟云学术

Evaluation of the Amplex eazyplex Loop-Mediated Isothermal Amplification Assay for Rapid Diagnosis of Pneumocystis jirovecii Pneumonia

Published:2020-11-18 Issue:12 Volume:58 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Huber Timo¹,Serr Annerose²,Geißdörfer Walter¹,Hess Christina²,Lynker-Aßmus Christian¹,von Loewenich Friederike D.³,Bogdan Christian¹,Held Jürgen¹^ORCID

Affiliation:

1. Mikrobiologisches Institut-Klinische Mikrobiologie, Immunologie und Hygiene, Universitätsklinikum Erlangen und Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany

2. Department für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Freiburg, Freiburg, Germany

3. Department of Medical Microbiology and Hygiene, Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany

Abstract

Quantitative PCR (qPCR) assays are the gold standard for diagnosis of Pneumocystis jirovecii pneumonia (PCP). However, they are laborious and require skilled personnel. Therefore, execution outside regular working hours of the molecular biology laboratory is limited. The eazyplex P. jirovecii assay (PJA) uses loop-mediated isothermal amplification for detection of P. jirovecii . It is performed directly with respiratory specimens, without the need for special skills, and delivers a result within 3 to 25 min.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01739-20

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