Evaluation of Blood Agar Microtiter Plates for Culturing Leishmania Parasites To Titrate Parasite Burden in Spleen and Peripheral Blood of Patients with Visceral Leishmaniasis-Reference-Cited by-同舟云学术

Evaluation of Blood Agar Microtiter Plates for Culturing Leishmania Parasites To Titrate Parasite Burden in Spleen and Peripheral Blood of Patients with Visceral Leishmaniasis

Published:2010-05 Issue:5 Volume:48 Page:1932-1934
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Maurya Radheshyam¹²,Mehrotra Sanjana¹,Prajapati Vijay Kumar¹,Nylén Susanne³,Sacks David³,Sundar Shyam¹

Affiliation:

1. Infectious Disease Research Laboratory, Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India

2. Department of Animal Sciences, University of Hyderabad, Hyderabad, India

3. Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892

Abstract

ABSTRACT Serial dilution of blood and spleen biopsy specimens, plated on Novy-MacNeal-Nicolle (NNN) blood agar using microtiter culture plates, is a sensitive and reproducible method for detection and growth of Leishmania parasites. Plates could be easily monitored, and growth could be rapidly detected. Moreover, parasite number may be estimated using this technique.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01733-09

Reference11 articles.

1. Belkaid, Y., S. Mendez, R. Lira, N. Kadambi, G. Milon, and D. Sacks. 2000. A natural model of Leishmania major infection reveals a prolonged “silent” phase of parasite amplification in the skin before the onset of lesion formation and immunity. J. Immunol.165:969-977.

2. Boelaert, M., K. Aoun, J. Liinev, E. Goetghebeur, and P. Van der Stuyft. 1999. The potential of latent class analysis in diagnostic test validation for canine Leishmania infantum infection. Epidemiol. Infect.123:499-506.

3. Chowdhury, M. S., A. el Harith, A. al Massum, E. al Karim, and A. al Rahman. 1993. Prevalence of agglutinating anti-Leishmania antibodies in two multi-thousand Bengali communities. Parasitol. Res.79:444-450.

4. Chulay, J. D., and A. D. Bryceson. 1983. Quantitation of amastigotes of Leishmania donovani in smears of splenic aspirates from patients with visceral leishmaniasis. Am. J. Trop. Med. Hyg.32:475-479.

5. Hide, M., R. Singh, B. Kumar, A. L. Bañuls, and S. Sundar. 2007. A microculture technique for isolating live Leishmania parasites from peripheral blood of visceral leishmaniasis patients. Acta Trop.102:197-200.

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