cis/trans Isomerase of Unsaturated Fatty Acids of Pseudomonas putida P8: Evidence for a Heme Protein of the Cytochrome c Type-Reference-Cited by-同舟云学术

cis/trans Isomerase of Unsaturated Fatty Acids of Pseudomonas putida P8: Evidence for a Heme Protein of the Cytochrome c Type

Published:1999-06 Issue:6 Volume:65 Page:2644-2649
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Holtwick Rita¹,Keweloh Heribert¹,Meinhardt Friedhelm¹

Affiliation:

1. Institut für Mikrobiologie, Westfälische Wilhelms-Universität Münster, 48149 Münster, Germany

Abstract

ABSTRACT From a pool of 600 temperature-sensitive transposon mutants of Pseudomonas putida P8, 1 strain was isolated that carries a mini-Tn 5 insertion within the cytochrome c operon. As a result, genes involved in the attachment of heme to cytochrome c -type proteins are turned off. Accordingly, cytochrome c could not be detected spectrophotometrically. The mutant also exhibited a remarkable reduction of cis-trans isomerization capability for unsaturated fatty acids. Consistent with the genetic and physiological data is the detection of a cytochrome c -type heme-binding motif close to the N terminus of the predicted polypeptide of the cis/trans isomerase ( cti ) gene ( C VA CH ; conserved amino acids in italics). The functional significance of this motif was proven by site-directed mutagenesis. A possible mechanism of heme-catalyzed cis-trans isomerization of unsaturated fatty acids is discussed.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.65.6.2644-2649.1999

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