Mechanism of Alanine Hyperproduction by Arthrobacter oxydans HAP-1: Metabolic Shift to Fermentation under Nongrowth Aerobic Conditions-Reference-Cited by-同舟云学术

Mechanism of Alanine Hyperproduction by Arthrobacter oxydans HAP-1: Metabolic Shift to Fermentation under Nongrowth Aerobic Conditions

Published:1999-06 Issue:6 Volume:65 Page:2781-2783
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Hashimoto Shin-ichi¹,Katsumata Ryoichi¹

Affiliation:

1. Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan

Abstract

ABSTRACT Arthrobacter oxydans HAP-1 hyperproduces dl -alanine in a non-growth-associated manner. We found that decreased activities of pyruvate dehydrogenase and of the enzyme catalyzing NADH oxidation in the stationary phase are paralleled by a shift of pyruvate metabolism to alanine synthesis by l -alanine dehydrogenase. We propose that this enzyme functions as an electron sink even under aerobic conditions.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.65.6.2781-2783.1999

Reference22 articles.

1. Studies on histidine fermentation.;Araki K.;Agric. Biol. Chem.,1971

2. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.;Bradford M. M.;Anal. Biochem.,1976

3. Calton G. J. The enzymatic preparation of l -alanine Biocatalytic production of amino acids and derivatives. Rozzell J. D. Wagner E. 1992 60 74 John Wiley & Sons Inc. New York N.Y

4. Overproduction of alanine by Arthrobacter strains with glucose-nonrepressible l-alanine dehydrogenase.;Hashimoto S.;Biotechnol. Lett.,1993

5. l-Alanine fermentation by an alanine racemase deficient mutant of the dl-alanine hyperproducing bacterium, Arthrobacter oxydans HAP-1.;Hashimoto S.;J. Ferment. Bioeng.,1998

Cited by 12 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Alanine dehydrogenases from four different microorganisms: characterization and their application in L-alanine production;Biotechnology for Biofuels and Bioproducts;2023-08-03

2. Metabolic engineering of microorganisms for L-alanine production;Journal of Industrial Microbiology and Biotechnology;2021-08-19

3. Amino Acid Production;The Prokaryotes;2013

4. Amino Acid Production by Corynebacterium glutamicum;Corynebacterium glutamicum;2012-07-02

5. Improvement of the Redox Balance Increases l-Valine Production by Corynebacterium glutamicum under Oxygen Deprivation Conditions;Applied and Environmental Microbiology;2011-12-02