Affiliation:
1. Department of Microbiology, College of Basic Sciences and Humanities, CCS Haryana Agricultural University, Hisar 125 004, India
Abstract
ABSTRACT
Electrotransformation of
Rhizobium leguminosarum
was successfully carried out with a 15.1-kb plasmid, pMP154 (Cm
r
), containing a
nodABC-lacZ
fusion by electroporation. The maximum transformation efficiency, 10
8
transformants/μg of DNA, was achieved at a field strength of 14 kV/cm with a pulse of 7.3 ms (186 Ω). The number of transformants was found to increase with increasing cell density, with no sign of saturation. In relation to DNA dosage, the maximum transformation efficiency (5.8 × 10
8
transformants/μg of DNA) was obtained with 0.5 μg of DNA/ml of cell suspension, and a further increase in the DNA concentration resulted in a decline in transformation efficiency.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
32 articles.
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