Author:
Ouwendijk Werner J. D.,Choe Alexander,Nagel Maria A.,Gilden Don,Osterhaus Albert D. M. E.,Cohrs Randall J.,Verjans Georges M. G. M.
Abstract
We analyzed the varicella-zoster virus (VZV) transcriptome in 43 latently infected human trigeminal ganglia (TG) with postmortem intervals (PMIs) ranging from 3.7 to 24 h. Multiplex reverse transcriptase PCR (RT-PCR) revealed no VZV transcripts with a PMI of <9 h. Real-time PCR indicated a significant increase (P= 0.02) in VZV ORF63 transcript levels but not the virus DNA burden with longer PMI. Overall, both the breadth of the VZV transcriptome and the VZV ORF63 transcript levels in human cadaver TG increased with longer PMI.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
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