Affiliation:
1. The Institute of Scientific and Industrial Research, Osaka University, Suita, Osaka, Japan
Abstract
Resting cells of
Corynebacterium
sp. E17 formed
O
-ethylhomoserine from ethyl alcohol for a few hours. Addition of
l
-homoserine greatly enhanced its formation. Thus, the formation of
O
-ethylhomoserine from ethyl alcohol by 27 bacteria, 6 yeasts, and 4 fungi was investigated by using growing cultures and resting cells in the presence of
l
-homoserine. The
O
-ethylhomoserine formed in the culture supernatant fluids or supernatant fluids of the reaction mixtures was identified by paper chromatography. Many organisms which were incapable of forming
O
-ethylhomoserine with growing cultures formed it with resting cells. The formation of
O
-ethylhomoserine appears to be restricted to strains of
Brevibacterium, Corynebacterium, Bacillus, Mycobacterium, Nocardia
, and
Streptomyces
.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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