Author:
Cevenini R,Donati M,Moroni A,Franchi L,Rumpianesi F
Abstract
Samples of nasopharyngeal secretions obtained from 70 infants and young children with acute respiratory disease were examined for the presence of respiratory syncytial virus by immunoperoxidase assay (IPA). The IPA was compared with the immunofluorescence assay and with cell culture isolation. Respiratory syncytial virus antigen-positive cells were detected by both IPA and immunofluorescence assay in 28 specimens; 25 samples were positive in cell culture. The agreement between virus isolation and IPA and IFA was 89%. The applicability of IPA to rapid viral diagnosis of respiratory syncytial virus infection is discussed.
Publisher
American Society for Microbiology
Reference12 articles.
1. A rapid immunoperoxidase assay for the detection of specific IgG antibodies to Chlamv-N-dia tr-riwh 0sotnti.s;Cevenini R.;J. Clin. Pathol.,1983
2. Detection of respiratory syncytial virus in nasal secretions from infants by enzyme-linked immunosorbent assay;Chao R. K.;J. Infect. Dis.,1979
3. Comparison of immunofluorescence and immunoperoxidase methods for viral diagnosis at a distance: a W.H.O. collaborative study;Gardner P. S.;Bull. W. H. O.,1978
4. Gardner P. S. and J. McQuillin. 1980. Rapid virus diagnosis. Application of immunofluorescence. Butterworth & Co. (Publishers). Ltd.. London.
5. The late detection of respiratory syncytial virus in cells of respiratory tract by immunofluorescence;Gardner P. S.;J. Hyg.,1970
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