Abstract
In an attempt to evaluate various factors that influence the growth of Mycobacterium lepraemurium in NC-5 medium, the effects of sulfur and --SH compounds were investigated. Cysteine could be replaced by equimolar concentrations of other --SH compounds containing carboxyl group, and at lower concentrations by nonpolar sulfhydryl compounds. The oxidized form of sulfhydryls, as well as certain organic and inorganic reducing agents, did not support growth. The results suggest that the function of sulfhydryl compounds is to maintain low reducing potential in the medium and also to participate in metabolic or biosynthetic pathways or both. A combination of dithiothreitol and thioglycolate gave better results than when these compounds were incorporated individually in the medium. This suggests the protective action of dithiothreitol in preventing oxidation of monothiols.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference20 articles.
1. Dithiothreitol, a new protective agent for SH groups;Cleland W. W.;Biochemistry,1964
2. Quantitative extraction of adenosine triphosphate from cultivable and host-grown microbes: calculation of adenosine triphosphate pools;Dhople A. M.;Appl. Microbiol.,1973
3. Factors that influence the growth of M. lepraemurium in the Nakamura system;Dhople A. M.;Int. J. Leprosy,1976
4. In vitro growth of M. lepraemurium, an obligate intracellular microbe;Dhople A. M.;Scie-nce,1977
5. Dhople A. M. and J. H. Hanks. 1977. The role of ultrasensitive determinations of ATP in investigating the physiology and growth of obligate intracellular microorganisms p. 251-283. In George A. Borun (ed.) Proceedings of the Second ATP Methodology Symposium San Diego Calif. SAI Technology Co. San Diego.
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献