Multiclonal Expansion of Klebsiella pneumoniae Isolates Producing NDM-1 in Rio de Janeiro, Brazil-Reference-Cited by-同舟云学术

Multiclonal Expansion of Klebsiella pneumoniae Isolates Producing NDM-1 in Rio de Janeiro, Brazil

Published:2017-04 Issue:4 Volume:61 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Aires Caio Augusto Martins¹,Pereira Polyana Silva¹,de Araujo Carlos Felipe Machado¹,Chagas Thiago Pavoni Gomes¹,Oliveira Jane Cleide Ribeiro²,Buonora Sibelle Nogueira³,Albano Rodolpho Mattos⁴,Carvalho-Assef Ana Paula D'Alincourt¹,Asensi Marise Dutra¹

Affiliation:

1. Laboratório de Pesquisa em Infecção Hospitalar, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil

2. Laboratório Central de Saúde Pública Noel Nutels, Rio de Janeiro, Brazil

3. Coordenação Estadual de Controle de Infecção Hospitalar, Secretaria Estadual de Saúde, Rio de Janeiro, Brazil

4. Departamento de Bioquímica, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil

Abstract

ABSTRACT We characterized NDM-1-producing Klebsiella isolates from Rio de Janeiro, Brazil. PCR was applied for resistance and virulence determinants. The genetic context of bla NDM was determined by S1 nuclease pulsed-field gel electrophoresis (PFGE) and hybridization. Genotyping was performed by PFGE and multilocus sequence typing (MLST). Most isolates carried multiple resistance genes and remained susceptible to amikacin, fosfomycin-trometamol, polymyxin B, and tigecycline. The spread of NDM-1-producing Klebsiella pneumoniae was not associated with clonal expansion and appears to be associated with Tn 3000 .

Funder

MCTI | Conselho Nacional de Desenvolvimento Científico e Tecnológico

Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.01048-16

Reference39 articles.

1. Characterization of a New Metallo-β-Lactamase Gene, bla NDM-1 , and a Novel Erythromycin Esterase Gene Carried on a Unique Genetic Structure in Klebsiella pneumoniae Sequence Type 14 from India

2. Does broad-spectrum -lactam resistance due to NDM-1 herald the end of the antibiotic era for treatment of infections caused by Gram-negative bacteria?