Affiliation:
1. EcoTopia Science Institute, Nagoya University, Nagoya 464-8603, Japan
Abstract
ABSTRACT
The purpose of this study was the enrichment and phylogenetic identification of bacteria that dechlorinate 4,5,6,7-tetrachlorophthalide (commercially designated “fthalide”), an effective fungicide for rice blast disease. Sequential transfer culture of a paddy soil with lactate and fthalide produced a soil-free enrichment culture (designated the “KFL culture”) that dechlorinated fthalide by using hydrogen, which is produced from lactate. Phylogenetic analysis based on 16S rRNA genes revealed the dominance of two novel phylotypes of the genus
Dehalobacter
(FTH1 and FTH2) in the KFL culture. FTH1 and FTH2 disappeared during culture transfer in medium without fthalide and increased in abundance with the dechlorination of fthalide, indicating their growth dependence on the dechlorination of fthalide.
Dehalobacter restrictus
TEA is their closest relative, with 97.5% and 97.3% 16S rRNA gene similarities to FTH1 and FTH2, respectively.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference36 articles.
1. Adrian, L., U. Szewzyk, J. Wecke, and H. Gorisch. 2000. Bacterial dehalorespiration with chlorinated benzenes. Nature408:580-583.
2. Bouchard, B., R. Beaudet, R. Villemur, G. McSween, F. Lépine, and J.-G. Bisaillon. 1996. Isolation and characterization of Desulfitobacterium frappieri sp. nov., an anaerobic bacterium which reductively dechlorinates pentachlorophenol to 3-chlorophenol. Int. J. Syst. Bacteriol.46:1010-1015.
3. Breitenstein, A., A. Saano, M. Salkinoja-Salonen, J. R. Andreesen, and U. Lechner. 2001. Analysis of a 2,4,6-trichlorophenol-dehalogenating enrichment culture and isolation of the dehalogenating member Desulfitobacterium frappieri TCP-A. Arch. Microbiol.175:133-142.
4. Bunge, M., L. Adrian, A. Kraus, M. Opel, W. G. Lorenz, J. R. Andreesen, H. Gorisch, and U. Lechner. 2003. Reductive dehalogenation of chlorinated dioxins by an anaerobic bacterium. Nature421:357-360.
5. Chida, T., T. Uekita, K. Stake, K. Hirano, K. Aoki, and T. Noguchi. 1982. Influence of fthalide in penetration process of Pyricularia oryzae. Nippon Shokubutsu Byori Gakkaiho48:58-63. (In Japanese.)
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