Author:
Cumberbatch N,Gurwith M J,Langston C,Sack R B,Brunton J L
Abstract
Ninety-six Aeromonas hydrophila isolates were tested for cytotoxin and hemolysin production. Sixty-six (69%) of the isolates were both cytotoxic and hemolytic, whereas the rest produced neither cytotoxin nor hemolysin. No evidence of a separate cytotonic activity could be found in any of the isolates. Cytotoxin activity correlated with enterotoxic activity. Of four cytotoxin-producing strains tested in the isolated rabbit ileal loop, three were definitely positive and one was borderline, whereas two nontoxigenic strains were negative. Cytotoxin activity appeared to be a stable property and could not be assoicated with any common identified plasmid; only 10 of 21 cytotoxin-producing strains could be shown to have any plasmid by agarose gel electrophoresis. Cytotoxin production correlated with a positive lysine decarboxylase phenotype (98%) or a positive Voges-Proskauer phenotype (94%), compared to 27% lysine decarboxylase-positive and 23% Voges-Proskauer-positive, cytotoxin-negative isolates (P less than 0.001 for both). In fecal samples, cytotoxin production correlated with diarrheal disease; of 40 diarrheal isolates, 32 (80%) were toxigenic compared to 9 (41%) of 22 nondiarrheal isolates (P = 0.004). It appears that A. hydrophila can be a cause of diarrhea and that this enteropathogenic potential is mediated by a cytotoxic enterotoxin.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference28 articles.
1. Enterotoxicity of Aeromonas hydrophila;Annapurna E.;J. Med. Microbiol.,1977
2. Effect of bacterial enterotoxins on the gastrointestinal tract;Banwell J. G.;Gastroenterology,1973
3. Partial characterization of aerolysin, a Iytic exotoxin from Aeromonas hydrophila;Bernheimer A. W.;Infect. Immun.,1974
4. Isolation of enterotoxigenic Aeromonas from fish;Boulanger R.;Can. J. Microbiol.,1977
5. Studies on Aeromonas and Plesiomonas species isolated from cases of choleraic diarrhoea;Chatterjee B. D.;Indian J. Med. Res.,1972
Cited by
176 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献