Gene Mapping of Rotavirus Double-Stranded RNA Segments by Northern Blot Hybridization: Application to Segments 7, 8, and 9-Reference-Cited by-同舟云学术

Gene Mapping of Rotavirus Double-Stranded RNA Segments by Northern Blot Hybridization: Application to Segments 7, 8, and 9

Published:1983-04 Issue:1 Volume:46 Page:317-320
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Dyall-Smith Michael L.¹,Azad Ahmed A.²,Holmes Ian H.¹

Affiliation:

1. School of Microbiology, University of Melbourne, Royal Parade, Melbourne, Australia

2. Division of Protein Chemistry, Commonwealth Scientific and Industrial Research Organization, Royal Parade, Melbourne, Australia

Abstract

Cloned DNA copies of double-stranded RNA segments 7, 8, and 9 of UK bovine rotavirus were nick-translated with [α- 32 P]ATP and hybridized to double-stranded RNA of various rotavirus strains which had been separated on long polyacrylamide gels and then transferred to o -aminophenylthioether paper. Specific hybridization of the UK calf clones to the separated RNA segments allowed the corresponding genes of four different rotaviruses to be rapidly determined.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.46.1.317-320.1983

Reference26 articles.

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3. Preparation and characterization of antisera to electrophoretically purified SAl 1 virus polypeptides;Bastardo J. W.;Infect. Immun.,1981

4. Gene-coding assignments of rotavirus double-stranded RNA segments 10 and 11;Dyall-Smith M. L.;J. Virol.,1981

5. Comparisons of rotavirus polypeptides by limited proteolysis: close similarity of certain polypeptides of different strains;Dyall-Smith M. L.;J. Virol.,1982

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