Affiliation:
1. Department of Biology, Yale University, New Haven, Connecticut 06510
Abstract
Forty tryptophan auxotrophs of
Bacillus subtilis
have been placed in six phenotypic classes on the basis of growth responses, accumulation properties, and, in some cases, specific enzymatic defects. Three-point transformation crosses between representative mutants of the six different types have permitted the determination of the orders of the gene loci. In addition, mutational site orders for mutants within each of the classes have been determined by the same techniques. The organization of the cluster of genes controlling tryptophan biosynthesis in
B. subtilis
appears to be essentially analogous to that of
Escherichia coli
and
Salmonella typhimurium
.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
18 articles.
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