Identification of Polysomal RNA in BHK Cells Infected by Sindbis Virus

Abstract

Polysomes were prepared from Sindbis virus-infected BHK cells. The major species of RNA in these polysomes was identified as 26 S RNA (interjacent RNA) by (i) disrupting the polysomes with EDTA; (ii) treating the infected cells with puromycin; and (iii) isolating polysomes from cells infected with a temperature-sensitive mutant that does not form nucleocapsids. Small amounts of 42 S RNA and 33 S RNA were also found in polysomes.