Promoter-probe plasmid for Bacillus subtilis-Reference-Cited by-同舟云学术

Promoter-probe plasmid for Bacillus subtilis

Published:1984-03 Issue:3 Volume:157 Page:965-967
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Donnelly C E,Sonenshein A L

Abstract

We have constructed a promoter-probe expression vector for Bacillus subtilis. This plasmid, pCED6, can be used to fuse various DNA sequences to the structural gene of Escherichia coli beta-galactosidase, permitting analysis of the promoter activity of such sequences. pCED6 replicates and confers drug resistances in both E. coli and B. subtilis.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.157.3.965-967.1984

Reference10 articles.

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4. Donnelly C. E. and A. L. Sonenshein. 1982. Genetic fusion of E. coli lac genes to a B. subtilis promoter p. 63-72. In A. Ganesan J. Hoch and S. Chang (ed.) Molecular cloning and gene regulation in bacilli. Academic Press Inc. New York.

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