Evaluation of 23S rRNA PCR Primers for Use in Phylogenetic Studies of Bacterial Diversity-Reference-Cited by-同舟云学术

Evaluation of 23S rRNA PCR Primers for Use in Phylogenetic Studies of Bacterial Diversity

Published:2006-03 Issue:3 Volume:72 Page:2221-2225
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Hunt Dana E.¹,Klepac-Ceraj Vanja¹,Acinas Silvia G.¹,Gautier Clement¹,Bertilsson Stefan¹,Polz Martin F.¹

Affiliation:

1. Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

Abstract

ABSTRACT The availability of a diverse set of 23S rRNA gene sequences enabled evaluation of the specificity of 39 previously published and 4 newly designed primers specific for bacteria. An extensive clone library constructed using an optimized primer pair resulted in similar gene richness but slightly differing coverage of some phylogenetic groups, compared to a 16S rRNA gene library from the same environmental sample.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.72.3.2221-2225.2006

Reference27 articles.

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4. Rapid Diagnosis of Bacteremia by Universal Amplification of 23S Ribosomal DNA Followed by Hybridization to an Oligonucleotide Array

5. Intraspecific Diversity of the 23S rRNA Gene and the Spacer Region Downstream in Escherichia coli

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