Evaluation of a Real-Time PCR Kit for Detecting Escherichia coli O157 in Bovine Fecal Samples-Reference-Cited by-同舟云学术

Evaluation of a Real-Time PCR Kit for Detecting Escherichia coli O157 in Bovine Fecal Samples

Published:2004-03 Issue:3 Volume:70 Page:1855-1857
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Bono James L.¹,Keen James E.¹,Miller Laura C.¹,Fox James M.¹,Chitko-McKown Carol G.¹,Heaton Michael P.¹,Laegreid William W.¹

Affiliation:

1. U.S. Meat Animal Research Center, Agricultural Research Service, U.S. Department of Agriculture, Clay Center, Nebraska 68933-0166

Abstract

ABSTRACT A commercially available real-time, rapid PCR test was evaluated for its ability to detect Escherichia coli O157. Both the sensitivity and specificity of the assay were 99% for isolates in pure culture. The assay detected 1 CFU of E. coli O157:H7 g −1 in artificially inoculated bovine feces following enrichment.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.70.3.1855-1857.2004

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