Acyl-acyl carrier protein is a donor of fatty acids in the NodA-dependent step in biosynthesis of lipochitin oligosaccharides by rhizobia

Author:

Ritsema T1,Lugtenberg B J1,Spaink H P1

Affiliation:

1. Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University, The Netherlands.

Abstract

NodA controls transfer of a fatty acid in the biosynthesis of lipochitin oligosaccharides by rhizobia. In an in vitro assay, we used de-N-acetylated chitin oligosaccharides substituted with an O-acetyl moiety as acyl acceptor substrates. We show that acyl-acyl carrier protein is used as a donor in NodA-directed fatty acid transfer.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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